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After the separation of protein or nucleic acids by electrophoresis, the molecule of interest is blotted onto a membrane. Specific RNA or DNA fragments are identified by hybridization with probes consisting of labeled DNA (Southern blotting) or RNA (Northern blotting) sequences. Labeled antibodies are used to probe for specific proteins in Western blotting, and fluorescence-based detection enables multiplex detection of several proteins simultaneously. Similar approaches can be applied to identify post-translational modifications of proteins (Eastern blotting), non-antibody protein-protein interactions (Far-Western blotting), protein-DNA interactions (Southwestern blotting), and protein-RNA interactions (Northwestern blotting).
There are other nucleic acid blotting methods, such as dot blots, slot blots, and colony/plaque lifts that do not include electrophoresis. Dot and slot blots involve direct application of the sample to the membrane, which is then probed. Colony and plaque lifts involve direct transfer of colonies of microorganisms from a culture plate onto a membrane which is probed.
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Whatman 3MM Chr, 31.5 x 33.5cm Cellulose Blotting Papers, 0.34 mm, 100 Pack, 3030-335Regular Price $223.00 Price $197.22 -
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Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 160 x 180 mm, 100 Pack, 10427813Regular Price $96.75 Price $76.63 -
Whatman Grade GB005 Gel Blotting Papers, Cellulose, 1.5 mm, 150 x 150 mm, 25 Pack, 10426972Regular Price $113.43 Price $89.84 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 300 x 600 mm, 25 Pack, 10426890Regular Price $143.49 Price $113.64 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 200 x 250 mm, 100 Pack, 10547922Regular Price $68.02 Price $53.87 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 580 x 600 mm, 50 Pack, 10426892Regular Price $425.00 Price $336.60 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 200 x 200 mm, 100 Pack,10427818Regular Price $130.11 Price $103.05 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 100 x 100 mm, 50 Pack, 10426880Regular Price $227.00 Price $179.78 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 150 x 200 mm, 100 Pack, 10427812Regular Price $98.43 Price $77.96 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 150 x 150 mm, 100 Pack, 10427810Regular Price $78.44 Price $62.12 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 70 x 100 mm, 100 Pack, 10427806Regular Price $25.17 Price $19.93 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 100 x 150 mm, 100 Pack, 10427805Regular Price $55.07 Price $43.61 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 110 x 140 mm, 100 Pack, 10427804Regular Price $30.84 Price $24.42 -
Whatman Grade GB005 Gel Blotting Papers, Cellulose, 1.5 mm, 580 x 580 mm, 25 Pack, 10426994Regular Price $573.00 Price $453.82 -
Whatman Grade GB005 Gel Blotting Papers, Cellulose, 1.5 mm, 200 x 200 mm, 25 Pack, 10426981Regular Price $140.14 Price $110.99 -
Whatman Grade GB003 Gel Blotting Papers, Cellulose, 0.8 mm, 460 x 570 mm, 100 Pack, 10427826Regular Price $765.00 Price $605.88