Qiagen CloneSaver Card, 96-Well Format, 5 Pack, WB120028

EluTrap System

The Elutrap system is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. Samples are purified with excellent recovery into volumes as low as 200 ul, without requiring sample pretreatment or special buffers.

The Elutrap system can be used with most horizontal gel electrophoresis chambers. The Elutrap electrophoresis chamber allows for the most efficient flow of current through the device and can be used for up to four samples simultaneously. Assembly of the Elutrap system is very easy. Gel slices are placed in the middle of the Elutrap device, which is then placed into a horizontal electrophoresis chamber. Molecules migrate from the gel slice into a trap area formed by BT1 and BT2 membranes.

Features:

• Versatile system can be used for nucleic acids and proteins.
• Purifies nucleic acids 14 bp to 15 kbp and proteins larger than Mr 3 to 5.
• No salt cushions or special buffers required for elution.
• Adjustable trap allows optimization of fil sample volume.
• Electrophoresis chamber holds up to four Elutrap devices simultaneously.

CloneSaver Card

The CloneSaver card by Qiagen is designed for the collection, long-term storage, and purification of plasmid and BAC DNA from bacterial clones in a 96-well format. It allows for the preparation of BAC and plasmid DNA with amazing ease. Apply 5µl bacterial culture, resuspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing. Bacteriophages are inactivated. DNA is easily accessible for downstream applications. Store up to 96 samples on each card and store sample DNA for years at room temperature. Plasmid DNA stored on CloneSaver cards is stable at room temperature for at least four years and counting.

Plasmid DNA can be eluted or used directly on a punch to transform bacteria either by electroporation or heat-shock methods. Immobilized plasmid DNA on a CloneSaver card punch can be used directly in a PCR. The PCR products remain in solution, do not bind to the punch, and are easily recoverable. Plasmid DNA can also be eluted for PCR or other studies. Plasmid DNA eluted from a CloneSaver punch can be amplified by rolling circle amplification, such as TempliPhi from GE Healthcare (formerly Amersham Biosciences) and then sequenced without the need for culture regrowth and plasmid purification. 5 Cards/Pack.